Monoclonal Antibody Recognition of Abscisic Acid Analogs

Monoclonal antibody recognition of abscisic Acid analogs.

Specificities of three monoclonal antibodies (15-I-C5, DBPA 1, and MAC 62) raised against the plant hormone (S)-(+)-abscisic acid (ABA) have been compared. Immunological cross-reactivities against fifteen biologically active analogs of ABA were measured. The ABA analogs were altered at one or more of four positions: the double bonds in the ring, at C-2 C-3 and at C-4 C-5, and in the oxidation l...

Conformational Analysis of Abscisic Acid Analogs Produced by Cercospora cruenta.

The conformation of hydroxy-γ-ionylideneacetic acids produced by Cercospora cruenta was examined by 'H-NMR analysis. (1)H-(1)H COSY and NOE data indicated that (2Z,4E,1'R,4'#)-4'-hydroxy-(γ)- ionylideneacetic acid has a cyclohexane ring of chair form furnishing a hydroxyl group and a 2,4-pentadienoic acid moiety, each with equatorial orientation, while (2Z,4E,1'S,3'S)-3'-hydroxy-γ-ionylideneace...

Kinetic Characterisation of a Single Chain Antibody against the Hormone Abscisic Acid: Comparison with Its Parental Monoclonal

A single-chain Fv fragment antibody (scFv) specific for the plant hormone abscisic acid (ABA) has been expressed in the bacterium Escherichia coli as a fusion protein. The kinetics of ABA binding have been measured using surface plasmon resonance spectrometry (BIAcore 2000) using surface and solution assays. Care was taken to calculate the concentration of active protein in each sample using in...

Development of a Novel Inhibitory Chimeric Anti-HER2 Monoclonal Antibody

Synthesis and Biological Activity of 2',3'-iso-Aryl-abscisic Acid Analogs.

2',3'-iso-Benzoabscisic acid (iso-PhABA), an excellent selective abscisic acid (ABA) analog, was developed in our previous work. In order to find its more structure-activity information, some structural modifications were completed in this paper, including the substitution of phenyl ring and replacing the ring with heterocycles. Thus, 16 novel analogs of iso-PhABA were synthesized and screened ...